摘 要

薄壳山核桃是一种优良的果材兼用型经济树种。采用四唑染色法对薄壳山核桃种子生活力进行测定，分析四唑的质量分数、染色温度以及染色时间对薄壳山核桃种子生活力测定的影响，并将生活力测定的结果与发芽试验结果进行比较分析。得出结论：

TTC溶液浓度,染色温度,染色时间三者之间存在一定的关系,通过改变三者之中的一者或两者可以达到改变其它两者或一者的目的。本文提出:温度35℃,浓度为0.5%,染色时间为8h是测定薄壳山核桃种子生活力的最佳条件。

关键词：薄壳山核桃；种子生活力；四唑。

Study on methods for rapid determination of seed viability of Carya illinoensis

ABSTRACT

Carya illinoensis is a good economy both nut and timber tree species. The seed viability of Pecan was determined by tetrazolium test. The paper analyses the influence of TTC solution concentration,dyeing temperature and dyeing time on the determination of Pecan seed viability. And the results of the determination of viability test and germination test results were compared and analyzed. It was found that:

There existed relationship among solution concentration, dyeing temperature and dyeing time. One or two of which could be practically altered to achieve the testing. This paper proposed that 0.5% of TTC concentration, 35℃ of temperature and 8h of dyeing time could be the best condition for seed viability testing of Carya illinoensis.

Key words: Carya illinoensis ; seed viability ; tetrazole.

目录

1 前言..............................................................................................................................................1

2 研究背景......................................................................................................................................2

2.1 薄壳山核桃简介..................................................................................................................2

2.2 种子生活力..................................................................................................................2

2.3 种子生活力快速测定的常用方法........................................................................................3

2.3.1 四唑测定法.................................................................................................................3

2.3.2 靛蓝测定法.................................................................................................................3

2.3.3 I²-KI测定法3............................................................................................................3

2.3.4 红墨水测定法.............................................................................................................3

2.3.5 离胚测定法.................................................................................................................3

2.3.6 过氧化氢（H₂O₂）鉴定法..................................................................................................4

2.3.7 溴麝香草酚兰法（BTB）...............................................................................................4

2.3.8 紫外线荧光法................................................................................................................4

2.3.9 外部形状目测法............................................................................................................4

2.3.10 X射线检验法...............................................................................................................4

2.3.11 失水率测定法..............................................................................................................5

2.4 薄壳山核桃种子生活力快速测定方法比较........................................................................5

3 四唑染色....................................................................................................................................6

3.1 四唑溶液的配制....................................................................................................................6

3.2 四唑溶液染色的原理............................................................................................................6

3.3 四唑染色的测定步骤............................................................................................................6

4 实验材料与方法............................................................................................................................8

4.1 实验材料 ..............................................................................................................................8